SPARCL™ (Spatial Proximity Analyte Reagent Capture Luminescence) assays use two specific antibodies; one conjugated to HRP, the other to acridan, a chemiluminescent substrate. When HRP and acridan conjugated antibodies bind to their target biomarker they are brought into close proximity. Upon addition of hydrogen peroxide (trigger solution), HRP catalyzes oxidation of proximal acridan molecules causing a flash of chemiluminescence that is proportional to biomarker concentration. Acridan molecules distant from HRP are not oxidized and therefore produce no luminescence. They also allow measurement of biomarkers in serum and plasma using a single 30-minute incubation that requires no wash steps.
Veterinary Biomarkers, Inc.
Veterinary Biomarkers provides non-GLP testing services for veterinary and academic research. All testing is performed using SPARCL™ immunoassays developed by Life Diagnostics, Inc. SPARCL™ assays allow rapid (30 minute) measurement of biomarker levels without the need for wash steps required by ELISA assays.
Assays are available for the following biomarker categories in species including, but not limited to, mouse, rat, cat, dog, pig, cow and horse.
- Cardiovascular (troponin-I, myoglobin)
- Acute phase proteins (CRP, haptoglobin, SAA, etc.)
- Immunoglobulins (IgA, IgE, IgG & IgM)
The VetBioI Machine
Tube Luminometer that is compact and super-sensitive. It integrates a built-in touch screen computer, and is equipped with 2 injectors. Light collection is maximized through the proximity of the detector to the sample and the use of optimized light reflectors surrounding it. Protocols for our SPARCL assays are preloaded, so it can be used as a standalone unit. It is perfect for use in a Veterinary Clinic.