Veterinary Biomarkers, Inc.
Our team has over 55 years of combined experience!
Veterinary Biomarkers Inc. is owned and operated by Chris and Laura Chadwick. With decades of experience in protein purification and immunoassay development, they have developed and validated all of the SPARCL™ assays listed on this site.
Veterinary Biomarkers was established in order to take advantage of SPARCL™ (Spatial Proximity Analyte Reagent Capture Luminescence)technology. SPARCL™ allows the development of rapid (30-minute) luminescence immunoassays that, unlike ELISA assays, do not require wash steps.
Diagnostic Testing and More
Veterinary Biomarkers manufactures assay kits, purified biomarkers and antibodies specifically for the veterinary diagnostics market.
SPARCL™ (Spatial Proximity Analyte Reagent Capture Luminescence) assays use two specific antibodies; one conjugated to HRP, the other to acridan, a chemiluminescent substrate. When HRP and acridan conjugated antibodies bind to their target biomarker they are brought into close proximity.
Upon addition of hydrogen peroxide (trigger solution), HRP catalyzes oxidation of proximal acridan molecules causing a flash of chemiluminescence that is proportional to biomarker concentration. Acridan molecules distant from HRP are not oxidized and therefore produce no luminescence.
The VetBio I
The VetBio I is a compact and sensitive single tube luminometer. Integrated with a built-in touch screen computer and two injectors, our machine is perfect for use in a veterinary setting.
Light collection is maximized through the proximity of the detector to the sample and the use of optimized light reflectors surrounding it. Protocols for our SPARCL™ assays are preloaded, so it can be used as a standalone unit.
Acute Phase Proteins
The acute phase response occurs when an inflammatory stimulus, such as injury, infection, or stress, causes macrophage/monocyte activation. Cytokines are then released, which trigger acute phase protein (APP) synthesis. Acute phase proteins such as; CRP, Haptoglobin, and SAA can be valuable prognostic tools. In humans APPs have been used to detect inflammation for decades; however, we have found that APPs are underutilized in veterinary medicine.